Fig. 1
HM inhibits colorectal adenocarcinoma HT29 and mCRC SW620 cell viability and proliferation. Cell viability was determined using MTT assay in HT29 (a) and SW620 (b) CRC cells that were cultured in 96-well plates for 24 h prior to treatment with various concentrations (5–200 μg/ml) of HM. The inhibitory effect of HM on CRC cell viability was observed after 24 h of treatment. The bar graphs show the percentage viability related to the control (corresponding to 100%) and the results are presented as mean ± standard deviation (SD) of three independent experiments. (*), (**), (***), and (****) signify a statistically significant difference (P < 0.05, P < 0.01, P < 0.001, and P < 0.0001) compared with the control. c The anti-proliferative effect of HM on HT29 cells was recorded using xCelligence RTDP for cell number measurement in real-time