Fig. 7

Both TLR4 downregulation and TLR4 blockade enhance HM-induced apoptosis while TLR4 blockade reduce HM-inhibited ERK phosphorylation. Representative Western blots showing downregulation of TLR4 expression detected in TLR4 siRNA-transfected HT29 (a) and SW620 (B) cells as compared with basal TLR4 expression level in untransfected cells (a, b). Representative scatter plots showing the percentage of apoptotic and necrotic cells in control untreated cells (top left), HM-treated cells (top right), TLR4 siRNA-transfected cells (bottom left), and in HM-treated TLR4 siRNA-transfected cells (bottom right) using HT29 (c) and SW620 (D) cells. Bar graphs showing the distribution of the percentage viable, early apoptotic, late apoptotic and necrotic cells in untreated cells, HM-, anti-TLR4 antibody (Ab)-, and HM added to anti-TLR4 Ab-treated HT29 (e) and SW620 (f) cells. Representative Western blot showing the expression of phospho-ERK (p-ERK) in untreated cells, HM-, anti-TLR4 antibody (Ab)-, and HM added to anti-TLR4 Ab-treated HT29 (g) and SW620 (h) cells