Fig. 5From: LBX2-AS1 up-regulated by NFIC boosts cell proliferation, migration and invasion in gastric cancer through targeting miR-491-5p/ZNF703LBX2-AS1 contributed to the course of GC via up-regulating ZNF703. A Bioinformatics presentation of the binding site between miR-491-5p and ZNF703. B ZNF703 expression was evaluated by RT-qPCR in GC cell lines and normal gastric cell. C RIP assay testified the enrichment of LBX2-AS1, miR-491-5p and ZNF703 in anti-Ago2 or anti-IgG group. D Luciferase activity of ZNF703-WT/Mut in transfected cells was detected by luciferase reporter assay. E RT-qPCR and western blot were carried out to detect the mRNA and protein expressions of ZNF703 in transfected cells. F Overexpressed ZNF703 was validated in RT-qPCR. Colony formation (G), EdU (H), flow cytometry (I), western blot (J) and transwell assays (K, L) were performed to measure GC cell proliferation, apoptosis and migration as well as invasion in differently transfected groups. **P < 0.01, ***P < 0.001Back to article page