Fig. 1
From: A novel circular RNA circENTPD7 contributes to glioblastoma progression by targeting ROS1
circENTPD7 is overexpressed in the glioblastoma tissue and cells. a The heatmap showed the representative dysregulated circRNAs in glioblastoma tissues analyzed by microarray. b CircENTPD7 (hsa_circ_0019421) was upregulated in the glioblastoma cells compared to the normal cells. c We could only amplify back-spliced forms of ENTPD7 from cDNA by PCR using divergent primers following by gel electrophoresis. The canonical linear forms of ENTPD7 in both and gDNA and were amplified in both cDNA and gDNA. d RT-qPCR for the abundance of circENTPD7 and ENTPD7 mRNA in glioblastoma cells treated with RNase R were performed, ENTPD7 mRNA rather than circENTPD7 were decreased after RNase R treatment. e CircENTPD7 was most localized in the cytoplasm by FISH analysis in U87 cells. f CircENTPD7 was enriched in U87 cytoplasm fraction. Levels of circENTPD7, ENTPD7 mRNA, GAPDH, and U6 RNA in purified U87 nuclear and cytoplasm fractions were detected by RT-qPCR. Data are presented as mean ± SD, Student’s t test, ***P < 0.001