Fig. 5
From: A novel circular RNA circENTPD7 contributes to glioblastoma progression by targeting ROS1
ROS1 serves as the target of miR-101-3p. a Diagram of 3′UTR of ROS1 containing wild type and mutations binding sites of miR-101-3p. b The expression of ROS1 in the glioblastoma were examined. c Expression of miR-101-3p mimics decreased luciferase activities in the cells transfected with plasmids containing wild type 3′UTR of ROS1, while expression of circENTPD7 increased luciferase activities in that; miR-101-3p_mut had no effect on the luciferase activity of ROS1 3′UTR. d miR-101-3p and circENTPD7 had no effect on the luciferase activity of ROS1 3′UTR_Mut; miR-101-3p_mut had no effect on the luciferase activity of circENTPD7. e Expression of miR-101-3p mimics decreased protein levels of ROS1 in glioblastoma cells. f Expression of circENTPD7 increased protein levels of ROS1 in glioblastoma cells. g Transwell assay demonstrated ROS1 was essential miR-101-3p inhibited cell migration. h Transwell assay demonstrated ROS1 was essential for circENTPD7 induced cell migration. Data are presented as mean ± SD, Student’s t test, **P < 0.01; ***P < 0.001