Fig. 2

GALNT7 is a target gene of miR-125a-5p in cervical cancer cell lines. a Bioinformatic analysis predicts the targeting site of miR-125a-5p in GALNT7 mRNA 3′-UTR. b The sequence alignments of GALNT7 in the wild-type and mutant-type were shown. c The luciferase reporter plasmid containing the WT or MUT GALNT7 3′-UTR was co-transfected into HEK293T cells with miR-125a-5p mimic or NC-mimic. The relative luciferase activity was tested at 48 h after co-transfection. d GALNT7 expression in adjacent normal and cervical cancer tissues were determined using RT-qPCR (n = 20). The mRNA e and protein f levels of GALNT7 in HeLa, SiHa, Caski and C33A cervical cell lines and NCEC were detected using RT-qPCR and western blot, respectively. The mRNA g and protein h levels of GALNT7 were detected after transfection with NC-mimic or miR-125a-5p mimic. *P < 0.05, **P < 0.01