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Fig. 2 | Cancer Cell International

Fig. 2

From: Silencing of microRNA-135b inhibits invasion, migration, and stemness of CD24+CD44+ pancreatic cancer stem cells through JADE-1-dependent AKT/mTOR pathway

Fig. 2

miR-135b inhibition could suppress viability, invasion, and migration and promote apoptosis in PCSCs. a CCK-8 assay was used to test the viability of PCSCs. b Flow cytometry was applied to detect apoptosis of PCSCs. c Western blot analysis was utilized to detect the expression of apoptotic proteins. d The cloning ability of PCSCs was tested by soft agar colony forming experiment. e onco-spheroids formation assay was used to detect the sphere-forming ability of PCSCs (×400). f Transwell assay was applied to examine the migration and invasion of PCSCs (×200). g Western blot analysis and RT-qPCR were used to detect expression of tumor stem cell markers. *p < 0.05 vs. the agomir-NC group; #p < 0.05 vs. the antagomir-NC group. The measurement data were expressed as the mean ± standard deviation. One-way ANOVA was applied for comparison among multiple groups, followed by Tukey’s post hoc test. Two-way ANOVA was performed to compare the data of cell viability among multiple groups at different time points. The experiment was repeated 3 times

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