Fig. 7

miR-135b activates AKT/mTOR pathway to promote cell proliferation and restrict apoptosis of PCSCs. a CCK-8 was used to test the proliferation of PCSCs. b Flow cytometry was applied to detect apoptosis. c Western blot analysis was utilized to examine the expression of apoptotic protein. d Clone forming assay was employed to determine the activity of PCSCs. e Onco-spheroids formation assay was used to test the sphere forming ability of PCSCs (×400). f Transwell was applied to detect the migration and invasion of PCSCs (×200). g Western blot analysis and RT-qPCR were carried out to detect expression of tumor stem cell markers. *p < 0.05 vs. the sh-NC group; ^#p < 0.05 vs. the DMSO group; ^&p < 0.05 vs. the agomir-miR-135b + DMSO group. The measurement data were expressed as the mean ± standard deviation. One-way ANOVA was applied for comparison among multiple groups, followed by Tukey’s post hoc test. Two-way ANOVA was performed to compare the data of cell viability among multiple groups at different time points. Repeated measurement ANOVA was used to compare the data among multiple groups at different time points, followed by the Bonferroni post hoc test. The experiment was repeated 3 times