Fig. 5From: Establishment and characterization of stable red, far-red (fR) and near infra-red (NIR) transfected canine prostate cancer cell linesa CT1258 and CT1258-mKate2C cell lines were cultured in serum-free medium. Various sizes of spheres formed after 10 days in culture. The displayed picture shows the well with a number of 128 initially seeded cells. The formed spheres were counted under the microscope, 50 µm diameter or bigger were counted as a sphere. b The cell surface marker CD49f was analyzed by flow cytometryBack to article page