Fig. 4

Regulatory effect of the IGBP1-AS1 on miR-24-1. a The predictive binding sites of IGBP1-AS1 with miR-24-1 was shown in the upper panel of the schematic diagram. The histogram presented the luciferase activities of the HCC70 cells. **P value < 0.01. b According to the anti-AGO2 RIP assays, the levels of IGBP1-AS1 and miR-24-1 were higher in anti-AGO2 compared to the anti-IgG immunoprecipitates. **P value< 0.01. c As shown in the scatter plot, the level of IGBP1-AS1 was higher in the miR-24-1-wt than in miR-24-1-mut. **P value < 0.01. d The expression level of miR-182 in BC cells with overexpressed IGBP1-AS1 relative to the cells with overexpressed-NC. The relative expression of IGBP1-AS1 (e) and cell proliferation (f) after transfection were assessed by the RT-qPCR and CCK-8 assay respectively. *P value < 0.05. g The BC cell invasion after transfection was evaluated with the transwell assay. *P value < 0.05