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Fig. 6 | Cancer Cell International

Fig. 6

From: LncRNA IGBP1-AS1/miR-24-1/ZIC3 loop regulates the proliferation and invasion ability in breast cancer

Fig. 6

a Predicted site where ZIC3 protein may bind to IGBP1-AS1 DNA. b RIP assay to identify the binding of IGBP1-AS1 to ZIC3. **P value < 0.01. c Upper Panel: Dual luciferase reporter assay to identify fluorescence intensity in cells with co-transfected truncated IGBP1-AS1 recombinant luciferase reporter vector and ZIC3 expression vector. **P value < 0.01; Lower Panel: dual luciferase reporter assay to identify fluorescence intensity of the cells with co-transfected mutated IGBP1-AS1 recombinant luciferase reporter vector and ZIC3 expression vector. **P value < 0.01. d ChIP assay to identify the binding ability of ZIC3 to the IGBP1-AS1 DNA binding site. **P value < 0.01. e Schematic representation of the regulation of the IGBP1-AS1/miR-24-1/ZIC3 loop in BC. IGBP1-AS1 up-regulates the level of ZIC3 via suppressing miR-24-1. On the other hand, the elevated ZIC3 synchronously promotes the expression of IGBP1-AS1 by binding to its DNA at promoter region, further inhibiting BC cell proliferation and invasion

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