Fig. 3

Knockdown of HOXD-AS1 inhibits the metastasis of HCC cell. A. The migration of HepG2 and SMMC-7721 cells after transfection of HOXD-AS1 siRNA was detected using wound healing assay. B. The invasion abilities of HepG2 and SMMC-7721 cells after transfection of HOXD-AS1 siRNA were detected by Transwell assay. C. The migration of BEL7402 and HuH7 cells after ectopic expression of HOXD-AS1 was measured using wound healing assay. D. The invasion abilities of BEL7402 and HuH7 cells after ectopic expression of HOXD-AS1 were analyzed. E. Downregulation of HOXD-AS1 increased the level of E-cadherin while reduced the level of N-cadherin. F. Downregulation of HOXD-AS1 increased the mRNA level of E-cadherin while reduced the mRNA level of N-cadherin. ^**P<0.01 compared to control. G. The stable HOXD-AS1 knockdown HepG2 cells were injected into nude mice, the micro-metastasis in the lung from the two groups were shown. H. The numbers of lung metastasis were quantified and showed by each data point. ^**P<0.01 compared to sh-NC