Fig. 6

SLC27A4 is regulated by HOXD-AS1 and miR-326. a Venn graph represented the candidate common target genes determined by four bioinformatics analysis tool (TargetScan, miRTarBase, StarBase and miRDB). b HepG2 or SMMC-7721 cells were transfected with miR-NC or miR-326 and the levels of SLC27A4 was measured by qPCR assay. ^**P< 0.01 compared to control. c The complementary sequences of miR-326 were discovered in 3’-UTR of SLC27A4 mRNA using TargetScan. The mutagenesis was performed in the complementary sites for the seed region of miR-326 (wt, wild type; mut, mutant type). MiR-326 inversely modulated the luciferase activity of plasmids that containing wt 3’-UTR of SLC27A4. ^**P< 0.01 compared to miR-NC + wt-SLC27A4. d HepG2 and SMMC-7721 cells were transfected with miR-326 or miR-NC. Western blotting assay indicated that up-regulation of miR-326 decreased the expression of SLC27A4. e The levels of SLC27A4 in HCC tissues and adjacent normal tissues were measured by qPCR. ^**P< 0.01 compared to normal. f The levels of SLC27A4 in four HCC cells and LO2 were detected by qPCR analysis. ^**P< 0.01 compared to LO2 cell. g The association between SLC27A4 and HOXD-AS1 in HCC tissues was evaluated