Fig. 7
From: Roles of eIF3m in the tumorigenesis of triple negative breast cancer
Influence of eIF3m knockdown on mitochondrial membrane potential and cell apoptosis in TNBC. a JC-1 green and JC-1 red of cells were observed in the fluorescence microscope (× 400); b JC-1 green and JC-1 red distributions were detected by flow cytometric analysis; c ratios of red/green fluorescence in mitochondria were measured through the microplate reader; d Blots and relative protein expression of Cyto-c expression in mitochondria and cytosol of stably transfected cells; e Cell apoptotic rates were detected by flow cytometry; f Apoptosis-related proteins of transfected cells were investigated via western blotting, Cle-Cas3: Cleaved-Caspase-3; Cas3: Caspase-3; Cle-Cas9: Cleaved-Caspase-9; Cas9: Caspase-9. *p < 0.05, **p < 0.01, ***p < 0.001