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Fig. 4 | Cancer Cell International

Fig. 4

From: A long non-coding RNA LINC00461-dependent mechanism underlying breast cancer invasion and migration via the miR-144-3p/KPNA2 axis

Fig. 4

Silencing LINC00461 inhibits cell invasion and migration in breast cancer via promoting miR-144-3p. si-NC and si-LINC00461 were transfected into MCF-7 and MDA-MB-157 cells. a qRT-PCR was performed to detect the miR-144-3p level and b RIP was conducted to validate the combination of miR-144-3p and LINC00461. Then, miR-144-3p inhibitor and inhibitor NC were simultaneously transfected into cells, contributing to si-NC + inhibitor NC, si-LINC00461 + inhibitor NC and si-LINC00461 + miR-144-3p inhibitor groups. All cells were collected for c qRT-PCR to detect transfection efficiency, then subjected to Transwell to determine the cell d, e invasion and f, g migration abilities, as well as to h Western blot to test the invasion and migration-related proteins N-cadherin, MMP-2, MMP-9 and E-cadherin (*p < 0.05)

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