Fig. 3
NEAT1 sequestered miR-206 by acting as a miR-206 sponge. a Schematic of the miR-206-binding sequence in NEAT1 and the mutant of the binding sequence. b Relative luciferase activity in SW1736 and KAT-18 cells cotransfected with WT-NEAT1 or MUT-NEAT1 and miR-NC mimic or miR-206 mimic. c NEAT1 enrichment by qRT-PCR in the RISC of SW1736 and KAT-18 cells transfected with miR-NC mimic or miR-206 mimic using anti-Ago2 or IgG antibody. d MiR-206 level by qRT-PCR in SW1736 and KAT-18 cells transfected with si-NC or si-NEAT1. The expression of miR-206 by qRT-PCR in 25 pairs ATC tissues and adjacent normal tissues (e), Nthy-ori3-1, SW1736 and KAT-18 cells (f), and SW1736 and KAT-18 cells exposed to 1% O2 for various time points (0, 3, 6, 12, 24 and 48 h) (g). h The correlation between NEAT1 level and miR-206 expression in ATC tissues using the Spearman test. *P < 0.05