Fig. 3

The association between miR-133b and TUG1. a The putative binding sites of TUG1 and miR-133b. b, c Luciferase activity was detected in SCC25/CDDP and CAL27/CDDP cells co-transfected with TUG1 WT or TUG1 MUT and miR-133b mimic or miR-NC. d The enrichment level of TUG1 was measured in SCC25/CDDP and CAL27/CDDP cells transfected with miR-133b mimic or miR-NC. e, f The expression of miR-133b was examined in cisplatin-resistant and sensitive TSCC cells by qRT-PCR. g The level of miR-133b was detected in SCC25/CDDP and CAL27/CDDP cells transfected with pcDNA, TUG1 overexpression vector, si-NC or si-TUG1. *P < 0.05