Skip to main content
Fig. 2 | Cancer Cell International

Fig. 2

From: LncRNA MIR4435-2HG triggers ovarian cancer progression by regulating miR-128-3p/CKD14 axis

Fig. 2

MIR4435-2HG knockdown inhibited the progression of OC cells. a The knockdown efficiency of MIR4435-2HG was verified using qRT-PCR. b, c Cell proliferation of SKOV3 and OVCAR3 cells with MIR4435-2HG knockdown in vitro was determined by MTT assay. d Cell apoptosis of SKOV3 and OVCAR3 cells after MIR4435-2HG knockdown was detected by flow cytometry assay. Q1: necrotic cells (AnnexinV-FITC)-/PI + ; Q2: late apoptotic or necrotic cells (AnnexinV + FITC) +/PI + ; Q3: early apoptotic cells (AnnexinV-FITC) +/PI-; Q4: unstained viable cells (AnnexinV-FITC)-/PI-. e, f The migration and invasion of SKOV3 and OVCAR3 cells after MIR4435-2HG knockdown were examined using transwell assay. g, h The wound healing assay was performed to observe the migration ratio in SKOV3 and OVCAR3 with MIR4435-2HG knockdown. i The protein levels of EMT markers (E-cadherin, Vimentin) and apoptosis indicators (Cleaved PARP, Bcl-2) in SKOV3 and OVCAR3 cells after MIR4435-2HG knockdown were analyzed by western blot. *P < 0.05

Back to article page