Fig. 8
From: LncRNA MIR4435-2HG triggers ovarian cancer progression by regulating miR-128-3p/CKD14 axis
MiR-128-3p abolished the effects of CDK14 on cell proliferation and apoptosis in OC cells. SKOV3 and OVCAR3 were transfected with CDK14, Vector, CDK14 + miR-128-3p mimics or CDK14 + NC mimics. a–c The expression of CDK14 was evaluated by qRT-PCR and western blot analysis. d, e Cell proliferation was detected by MTT assay. f The apoptosis rate of SKOV3 and OVCAR3 cells was measured by flow cytometry. Q1: necrotic cells (AnnexinV-FITC)-/PI + ; Q2: late apoptotic or necrotic cells (AnnexinV + FITC) +/PI+; Q3: early apoptotic cells (AnnexinV-FITC) +/PI−; Q4: unstained viable cells (AnnexinV-FITC)-/PI−. *P < 0.05