Fig. 5

MALAT1 targets miR-146a. a As shown in bioinformatic analysis, miR-146a has a binding site in MALAT1. b DLRA was subsequent to simultaneous transfection of a luciferase reporter with a wild-type or mutant from MALAT1, and miR-146a mimics/NC into HEK293T cells. c Q-PCR analysis illustrated downregulated miR-146a levels in HCC specimens from patients (n = 40) and healthy volunteers (n = 12). Correlation analysis of tumor tissues from 40 HCC cases between MALAT1 and miR-146a expression (P < 0.001). d Q-PCR displayed how miR-146a was expressed in HepG2, Huh-7, and normal human hepatic cells. e, f miR-146a levels in HepG2/Huh-7 cells, which underwent siMALAT1 and siNC transfection, were detected using Q-PCR. Data are shown as mean ± SD. *P < 0.05, **P < 0.01, and ***P < 0.001, when in contrast with the indicated group