Skip to main content
Fig. 2 | Cancer Cell International

Fig. 2

From: Long non-coding RNA OIP5-AS1 suppresses multiple myeloma progression by sponging miR-27a-3p to activate TSC1 expression

Fig. 2

Overexpression of OIP5-AS1 inhibited proliferation and metastasis, but promoted apoptosis of MM cells. MM NCI-H929 and MM1.S cells were transfected with LncRNA OIP5-AS1 or Lnc-NC. a The relative expression of lncRNA OIP5-AS1 in transfected cells was analyzed by qRT-PCR assay. b Cell viability of transfected cells was analyzed by CCK-8 assay. c The colony formation ability of transfected cells was monitored via colony formation assay. d, e The apoptosis rate and cell cycle distribution were tested through flow cytometry assay. f, g The migration and invasion abilities of transfected cells were examined by transwell assay. h, i The protein levels of Cleaved caspase 3/total caspase 3, Cleaved caspase 1, γ-H2AX, Cyclin D1, p21, Ki-67, MMP9, MMP7 and MMP10 were evaluated by western blot analysis. *P < 0.05

Back to article page

Cancer Cell International

ISSN: 1475-2867

Contact us