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Fig. 3 | Cancer Cell International

Fig. 3

From: Long non-coding RNA OIP5-AS1 suppresses multiple myeloma progression by sponging miR-27a-3p to activate TSC1 expression

Fig. 3

LncRNA OIP5-AS1 directly targeted miR-27a-3p. a The binding sites between OIP5-AS1 and miR-27a-3p as well as the mutant were shown. b Dual-luciferase reporter assay was carried out to measure the luciferase activity in LncRNA OIP5-AS1-wt group and LncRNA OIP5-AS1-mut group of NCI-H929 and MM1.S cells. c The levels of OIP5-AS1 and miR-27a-3p were evaluated after Ago2 or IgG RIP by qRT-PCR assay. d The expression of miR-27a-3p in NCI-H929 and MM1.S cells transfected with Lnc-NC, LncRNA OIP5-AS1, si-NC or si-LncRNA OIP5-AS1 was analyzed by qRT-PCR assay. e The relative expression of miR-27a-3p in bone marrows of 38 MM patients and 25 healthy donors was tested by qRT-PCR assay. f The correlation between the expression of OIP5-AS1 and miR-27a-3p was determined via Pearson correlation analysis. *P < 0.05

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Cancer Cell International

ISSN: 1475-2867

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