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Fig. 4 | Cancer Cell International

Fig. 4

From: Long non-coding RNA OIP5-AS1 suppresses multiple myeloma progression by sponging miR-27a-3p to activate TSC1 expression

Fig. 4

Upregulation of miR-27a-3p counteracted the effects of OIP5-AS1 on the cellular behaviors of MM cells. MM NCI-H929 and MM1.S cells were transfected with Lnc-NC, LncRNA OIP5-AS1, LncRNA OIP5-AS1 + NC or LncRNA OIP5-AS1 + miR-27a-3p. a The relative expression of miR-27a-3p in transfected cells was detected by qRT-PCR assay. b Cell viability of transfected cells was analyzed by CCK-8 assay. c The colony formation ability of transfected cells was measured via colony formation assay. d, e The apoptosis rate and cell cycle distribution were detected through flow cytometry assay. f, g The migration and invasion abilities of transfected cells were evaluated by transwell assay. h, i Protein levels of Cleaved caspase 3/total caspase 3, Cleaved caspase 1, γ-H2AX, Cyclin D1, p21, Ki-67, MMP9, MMP7 and MMP10 were examined by western blot analysis. *P < 0.05

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