Fig. 5

MiR-27a-3p could target TSC1 in MM. a The binding sites between miR-27a-3p and TSC1 3′UTR, as well as the mutant were exhibited. b Dual-luciferase reporter assay was carried out to measure the luciferase activity in TSC1-wt group and TSC1-mut group of NCI-H929 and MM1.S cells. c The levels of miR-27a-3p and TSC1 were examined after Ago2 or IgG RIP by qRT-PCR assay. d The expression of TSC1 protein in NCI-H929 and MM1.S cells transfected with NC, miR-27a-3p, anti-NC or anti-miR-27a-3p was tested by western blot assay. e The expression of TSC1 protein in NCI-H929 and MM1.S cells transfected with Lnc-NC, LncRNA OIP5-AS1, LncRNA OIP5-AS1 + NC or LncRNA OIP5-AS1 + miR-27a-3p was measured by western blot assay. f, g The mRNA and protein levels of TSC1 in bone marrows of 38 MM patients and 25 healthy donors were determined by qRT-PCR and western blot assays, respectively. h, i The correlation between the expression of TSC1 mRNA and OIP5-AS1, as well as between the expression of miR-27a-3p and TSC1 mRNA was determined via Pearson correlation analysis. ^*P < 0.05