Fig. 2

Hsa_circ_0068307 acts as a sponge for miR-147, and c-Myc is a direct target of miR-147. a The complementary sites within hsa_circ_0068307 and miR-147 were predicted by bioinformatics analysis. The mutated (Mut) version of hsa_circ_0068307 is also shown. b Dual luciferase reporter assays demonstrated that miR-147 is a direct target of hsa_circ_0068307. Data are presented as the mean ± SD. ***P < 0.001 vs. control. c, d qRt-PCR detection showing the expression of hsa_circ_0068307 and miR-147 in T24 and UMUC3 cells transfected with si-circRNA or miR-147 inhibitor. Data are presented as the mean ± SD. ***P < 0.001 vs. NC. ^###P < 0.001 vs. si-circRNA. e The predicted binding sites of miR-147 with the 3′-UTR of c-Myc. The mutated version of the 3′-UTR-c-Myc is also shown. f Relative luciferase activity was determined 48 h after transfection with miR-147 mimic/normal control or with the 3′-UTR-c-Myc wild-type/Mut in HEK293T cells. Data are presented as the mean ± SD. ***P < 0.001 vs. control. g, h qRt-PCR detection showing the expression of miR-147 (g) and c-Myc (h) in T24 and UMUC3 cells transfected with miR-147 mimic. Data are presented as the mean ± SD. ***P < 0.001 vs. NC