Fig. 3

Knockdown of hsa_circ_0068307 inhibits cancer stem cell-mediated BCa cell proliferation and migration by regulating the miR-147/c-Myc axis in in vitro. a, b RT-qPCR assay showing hsa_circ_0068307 expression in T24 (a) and UMUC3 (b) cells after transfection with siRNA against hsa_circ_0000291, combined with or without miR-147 inhibitor or the c-Myc overexpression vector. Data are presented as the mean ± SD. ***P < 0.001 vs. NC. c, d RT-qPCR assay showing miR-147 expression in T24 (c) and UMUC3 (d) cells. Data are presented as the mean ± SD. ***P < 0.001 vs. NC. ^###P < 0.001 vs. si-circRNA. e, f RT-qPCR detection showing c-Myc expression in T24 (e) and UMUC3 (f) cells. Relative protein levels were analyzed and data are presented as the mean ± SD. ***P < 0.001 vs. NC. ^###P < 0.001 vs. si-circRNA. g, h CCK8 assays were performed to assess cell proliferation in T24 (g) and UMUC3 (h) cells. Data are presented as the mean ± SD. *P < 0.05, ***P < 0.001 vs. NC. ^###P < 0.001 vs. si-circRNA. i, j Clone formation assay showing the proliferation of T24 and UMUC3 cells. Data are presented as the mean ± SD. **P < 0.01, ***P < 0.001 vs. NC. ^###P < 0.001 vs. si-circRNA. k, l Cell migration was determined in T24 and UMUC3 cells using Transwell^® assays. Data are presented as the mean ± SD. **P < 0.01, ***P < 0.001 vs. NC. ^###P < 0.001 vs. si-circRNA. m Western blow analysis showing the expression of cancer stem cell-related proteins OCT-4, Sox-2, and NANOG in T24 and UMUC3 cells. Relative protein levels were analyzed and data are presented as the mean ± SD. ***P < 0.001 vs. NC. ^###P < 0.001 vs. si-circRNA