Fig. 1

The expression of circ_100565 in NSCLC tissues and cells. a Heat map: differential expression of circRNAs in NSCLC tissues (NSCLC) and adjacent normal tissues (Normal). b Circ_100565 was markedly upregulated by more than 2.99-fold in NSCLC compared with that in Normal. c–h QRT-PCR was used to measure the first 3 up-regulated circRNAs and first 3 down-regulated circRNAs in 10 pairs of NSCLC tissues and normal tissues (NC). i The expression of circ_100565 in NSCLC and Normal was detected by qRT-PCR. j Kaplan–Meier analysis examined the correlation between circ_100565 expression and overall survival of NSCLC patients. k Circ_100565 expression in NSCLC cell lines (Calu-3, Calu-6, A549 and H1299) and HBE1 cells was determined by qRT-PCR. l The expression levels of circ_100565 and GAPD in A549 and H1299 cells were assessed by qRT-PCR to evaluate the authenticity of circ_100565. m Subcellular fractionation assay was used to measure the expression levels of circ_100565, U6 and GAPDH in the nuclear and cytoplasm of A549 and H1299 cells. *P < 0.05, **P < 0.01, ***P < 0.001