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Fig. 2 | Cancer Cell International

Fig. 2

From: Circ_100565 promotes proliferation, migration and invasion in non-small cell lung cancer through upregulating HMGA2 via sponging miR-506-3p

Fig. 2

Effects of circ_100565 on the progression of NSCLC cells. A549 and H1299 cells were transfected with sh-circ_100565#1/2 or con. a The expression of circ_100565 was detected by qRT-PCR to evaluate transfection efficiency. b, c CCK-8 assay was performed to assess the viability of A549 and H1299 cells. d Colony formation assay was used to measure the number of colonies in A549 and H1299 cells. e The cell cycle distribution of A549 and H1299 cells was evaluated using flow cytometry. f, g The numbers of migrated and invaded A549 and H1299 cells were determined by transwell assay. h, i The protein levels of PCNA and MMP9 in A549 and H1299 cells were detected by WB analysis. j WB analysis was performed to measure the protein level of E-cadherin and Vimentin in A549 and H1299 cells. * P < 0.05, **P < 0.01

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