Fig. 5

HMGA2 was a target of miR-506-3p. a The expression changes of 10 targeted genes in A549 cells transfected with smiR-506-3p or miR-NC were detected by qRT-PCR. b The sequences of miR-506-3p contained the HMGA2 3′UTR binding sites or mutant binding sites were shown. The interaction between miR-506-3p and HMGA2 in A549 or H1299 cells was assessed by dual-luciferase reporter assay (c) and biotin-labeled pull down (d). e The expression of miR-506-3p was detected by qRT-PCR to evaluate the transfection efficiency of miR-506-3p mimic. f The effect of miR-506-3p mimic on the mRNA expression of HMGA2 was measured by qRT-PCR in A549 and H1299 cells. g The effect of miR-506-3p mimic on the protein level of HMGA2 was detected by WB analysis in A549 and H1299 cells. h, i The mRNA and protein expression levels of HMGA2 in NSCLC tissues (NSCLC) and adjacent normal tissues (Normal) were determined by qRT-PCR and WB analysis. j The correlation between HMGA2 and miR-506-3p or circ_100565 expression was detected by Pearson correlation analysis. *P < 0.05, **P < 0.01, ***P < 0.001