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Fig. 6 | Cancer Cell International

Fig. 6

From: Circ_100565 promotes proliferation, migration and invasion in non-small cell lung cancer through upregulating HMGA2 via sponging miR-506-3p

Fig. 6

Effects of miR-506-3p and HMGA2 on the progression of NSCLC cells. A549 and H1299 cells were transfected with sh-circ_100565#2 and anti-miR-506-3p or HMGA2 overexpression plasmid. a The protein level of HMGA2 was measured by WB analysis to evaluate the transfection efficiency of HMGA2 overexpression plasmid. b The expression of miR-506-3p was detected by qRT-PCR to evaluate the transfection efficiency of anti-miR-506-3p. c, d The viability of A549 and H1299 cells was determined by CCK-8 assay. e Colony formation assay was performed to detect the number of colonies in A549 and H1299 cells. f, g Transwell assay was used to assess the numbers of migrated and invaded A549 and H1299 cells. h, i WB analysis was carried out to measure the protein levels of PCNA and MMP9 in A549 and H1299 cells. * P < 0.05, **P < 0.01

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