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Fig. 2 | Cancer Cell International

Fig. 2

From: LncRNA FLVCR1-AS1 promotes proliferation, migration and activates Wnt/β-catenin pathway through miR-381-3p/CTNNB1 axis in breast cancer

Fig. 2

MYC stimulates FLVCR1-AS1 transcription. a qRT-PCR analysis was used to determine the expression of MYC after transfecting with pcDNA3.1 and pcDNA3.1/MYC plasmid. b qRT-PCR detected the expression of FLVCR1-AS1 after MYC overexpression. c qRT-PCR was used to determine expression of MYC after transfecting with sh-Ctrl and sh-MYC. d The expression of FLVCR1-AS1 was detected after MYC knockdown. e ChIP assay using anti-MYC and anti-IgG was performed to explore the affinity of MYC for FLVCR1-AS1 promoter. f HEK-293T was co-transfected with sh-Ctrl, sh-MYC, pcDNA3.1 and pcDNA3.1/MYC vector and a luciferase reporter for 48 h was performed. g Potential MYC binding site with FLVCR1-AS1 promoter region was detected on Jaspar. H. ChIP assay was performed to determine the specific sequence in FLVCR1-AS1 promoter for the binding with MYC. i Luciferase reporter was conducted to investigate the effect of MYC on FLVCR1-AS1 transcription activity in SKBR3 and MCF7 cells. **P < 0.01

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