Fig. 1

HE4 protein expression levels in the four experimental groups of control (Ctrl), HE4 overexpression (HE4), Pac treatment (Pac), and overexpression plus Pac treatment (HE4 + Pac). Western blotting was performed with HE4- and GAPDH-specific antibodies, respectively, following cell transfection with HE4 expression plasmid DNA and/or Pac treatment. a Representative results of the Western blotting analysis for HE4 and GAPDH. b The results were scanned for densitometry analysis. Levels of HE4 were normalized with those of GAPDH, the protein loading marker. A more than three-fold increase in HE4 level was achieved by the overexpression manipulation. Note that HE4 levels remained stable following Pac treatment