Fig. 3

HE4 overexpression decreased the Capan-1 and Suit-2 cell sensitivity to Pac. Following cell transfection with HE4-expressing plasmid and Pac treatment, cell numbers of the four experimental groups were counted every 24 h. a Pac inhibited cell proliferation at each time point of post-treatment, but HE4 overexpression promoted cell proliferation. (Left Panel: Capan-1 cell line; Right panel: Suit-2 cell line). b The two curves showing B/A (Pac⁺/Pac⁻, without HE4 overexpression) and D/C (Pac⁺/Pac⁻, with HE4 overexpression) values became separate when Pac treatment proceeded, indicating an effect by HE4 overexpression on cell sensitivity to Pac in both cell lines (Left Panel: Capan-1 cell line; Right panel: Suit-2 cell line). c Value X = (D/C)/(B/A) was significantly higher than 1 compared with the control, indicating that HE4 overexpression decreased the cell sensitivity to paclitaxel, and HE4 and Pac effects were interactive (Left Panel: Capan-1 cell line; Right panel: Suit-2 cell line). *p < 0.05; ** p < 0.01