Fig. 6

HE4 overexpression and Pac treatment affected the expression of cell cycle regulators PCNA and p21. a (Capan-1 cell line), and b (Suit-2 cell line), representative results of Western blotting with the use of specific antibodies against PCNA, p21, or GAPDH, respectively, for the four groups of control, HE4, Pac, and HE4 + Pac. c (Capan-1 cell line) and d (Suit-2 cell line), results of densitometry analysis showing the relative protein levels of PCNA (left panel) or P21 (right panel), respectively. e (Capan-1 cell line) and f (Suit-2 cell line), the significant changes of X values for PCNA (left panel) or p21 (right panel) with HE4 overexpression indicated interactive effects between HE4 overexpression and paclitaxel treatment. *p < 0.05; ** p < 0.01