Biological effects of selective COX-2 inhibitor NS398 on human glioblastoma cell lines

Table 1 Immunophenotypic characterization of GBM adherent cells and relative neurosphere

β-tubulin	Sox-2	Nestin	GFAP
U87MG adherent cells
% positive cells
51.30 ± 4.21	50.57 ± 3.02	92.41 ± 6.84	69.91 ± 2.38
MFI
45.21 ± 3.78	36.57 ± 2.47	249.38 ± 15.86	139.14 ± 11.70
U87MG neurospheres
% positive cells
89.47 ± 7.23	95.41 ± 8.21	96.31 ± 4.39	59.71 ± 3.40
MFI
210.60 ± 10.30	129.77 ± 10.82	511.61 ± 34.90	60.69 ± 5.11
T98G adherent cells
% positive cells
80.52 ± 4.34	86.84 ± 6.40	89.40 ± 6.96	52.96 ± 4.01
MFI
117.51 ± 9.87	44.58 ± 36.84	99.58 ± 7.43	58.88 ± 1.99
T98G neurospheres
% positive cells
89.88 ± 5.73	97.63 ± 7.70	96.64 ± 4.80	4.11 ± 0.98
MFI
189.40 ± 13.85	90.03 ± 8.02	411.38 ± 36.11	28.00 ± 1.45

Percentage (%) and value of Median Fluorescence Intensity (MFI) of β-tubulin III, SOX-2, nestin and GFAP in U87MG and T98G maintained in standard culture conditions (adherent cells) and in DMEM/F12 medium serum free with EGF, b-FGF and B27 supplement for neurosphere generation. Data are from one representative out of two independent experiments in duplicates ± SD