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Table 1 Immunophenotypic characterization of GBM adherent cells and relative neurosphere

From: Biological effects of selective COX-2 inhibitor NS398 on human glioblastoma cell lines

β-tubulin Sox-2 Nestin GFAP
U87MG adherent cells
 % positive cells
  51.30 ± 4.21 50.57 ± 3.02 92.41 ± 6.84 69.91 ± 2.38
 MFI
  45.21 ± 3.78 36.57 ± 2.47 249.38 ± 15.86 139.14 ± 11.70
U87MG neurospheres
 % positive cells
  89.47 ± 7.23 95.41 ± 8.21 96.31 ± 4.39 59.71 ± 3.40
 MFI
  210.60 ± 10.30 129.77 ± 10.82 511.61 ± 34.90 60.69 ± 5.11
T98G adherent cells
 % positive cells
  80.52 ± 4.34 86.84 ± 6.40 89.40 ± 6.96 52.96 ± 4.01
 MFI
  117.51 ± 9.87 44.58 ± 36.84 99.58 ± 7.43 58.88 ± 1.99
T98G neurospheres
 % positive cells
  89.88 ± 5.73 97.63 ± 7.70 96.64 ± 4.80 4.11 ± 0.98
 MFI
  189.40 ± 13.85 90.03 ± 8.02 411.38 ± 36.11 28.00 ± 1.45
  1. Percentage (%) and value of Median Fluorescence Intensity (MFI) of β-tubulin III, SOX-2, nestin and GFAP in U87MG and T98G maintained in standard culture conditions (adherent cells) and in DMEM/F12 medium serum free with EGF, b-FGF and B27 supplement for neurosphere generation. Data are from one representative out of two independent experiments in duplicates ± SD