Fig. 4From: N-alkylisatin-based microtubule destabilizers bind to the colchicine site on tubulin and retain efficacy in drug resistant acute lymphoblastic leukemia cell lines with less in vitro neurotoxicityDisplacement of MTC from the colchicine-binding site by a HMB-isatin, b I4M-isatin and c N1M-isatin. MTC fractional saturation values were calculated based on the concentration of MTC binding sites (0.8 per tubulin dimer) and the MTC binding constant (4.7 × 105 M−1). Solid line is the best fit to a single site competition model as calculated by EQUIGRA5. Values are the mean of triplicates ± SEMBack to article page