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Fig. 5 | Cancer Cell International

Fig. 5

From: N-alkylisatin-based microtubule destabilizers bind to the colchicine site on tubulin and retain efficacy in drug resistant acute lymphoblastic leukemia cell lines with less in vitro neurotoxicity

Fig. 5

Effect of N-alkylisatins and Vinca alkaloids on SH-SY5Y neurite outgrowth and number of neurite branch points. SH-SY5Y neuroblastoma cells were incubated with 10 μM retinoic acid for 96 h to induce differentiation of cells into a mature dopaminergic-like neurotransmitter phenotype. Cells were then treated with increasing concentrations (1–100 nM) of N-alkylisatin (HMB-isatin, I4M-isatin or N2M-isatin) or Vinca alkaloid (vincristine; VCR or vinblastine; VBL) for 48 h. Changes in a neurite length or b number of neurite branch points were analyzed using the IncuCyte ZOOM, and measured using NeuroTrack analysis software. Mean ± SD, n = 3. NS = not significant, *P < 0.001,#P < 0.01

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