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Table 2 Binding affinity values for HMB-isatin, I4M-isatin and N1M-isatin compared with reference compounds of the colchicine site

From: N-alkylisatin-based microtubule destabilizers bind to the colchicine site on tubulin and retain efficacy in drug resistant acute lymphoblastic leukemia cell lines with less in vitro neurotoxicity

Compound Binding affinity (M−1) ± SEMa
Colchicine 1.16 × 107 (37 °C) [70]
Podophyllotoxin 1.8 × 106 [71]
MTC 4.7 × 105 [72]
HMB-isatin 2.7 ± 1.1 × 105
I4M-isatin 3.4 ± 1.5 × 105
N1M-isatin 1.4 ± 0.2 × 105(× 104)
  1. aValues (Kb) are the mean of three independent experiments performed in triplicate (n = 9) ± SEM