Fig. 1

 ESRP1 induced MET, inhibited invasion and migration, promoted colonization of EOC cells. a ESRP1 expression in ESRP1-SKOV3 and ESRP1-A2780 cells was confirmed by qRT-PCR and western blot. GAPDH served as a control. b ESRP1-SKOV3, ESRP1-A2780 and the control groups (EV-SKOV3, EV-A2780) migration and invasion assay using a Corning Transwell System. Scale bar 100 μm. Histogram represented cell count. c ESRP1-SKOV3 and EV-SKOV3 cells clone forming assay. Histogram showed the count of cell colonies after cultured for 14 days. d Epithelial markers E-Cadherin, ZO-1 and mesenchymal markers Vimentin, Fibronectin were detected in ESRP1-SKOV3 and ESRP1-A2780 compared to the control groups (EV-SKOV3, EV-A2780) by qRT-PCR. e ESRP1 was positively correlated with E-Cadherin and negatively correlated with VIM in dataset GSE18520. f Kaplan–Meier curve showed overall survival of patients with ovarian cancer without residual disease in stage III and IV in TCGA database. High level ESRP1 group (red, n = 39) or low level ESRP1 group (blue, n = 39). g ESRP1/EV-SKOV3 cells were injected into the abdominal cavity of nude mice to construct a orthotopic xenograft model of ovarian cancer. Red circles marked macroscopic tumor tissue. h Histograms showed the tumor weight and volume in nude mice of experimental and control groups. *P<0.05, **P<0.01, ***P<0.001. Error bars indicate SEM