Fig. 2

circ-0005585 bound to miRs targeting ESRP1. a miR binding sites in ESRP1 3’ UTR. b circRNAs (blue diamonds) compete with miRs (red circles) to regulate ESRP1. c Luciferase reporter assay was used to screen individual miR that may regulate ESRP1 and the binding sites of ESRP1 3’UTR to miRs: miR-23a/b and miR-15a/15b/16. d Dual luciferase reporter assay validated the inhibition of miR-23a/b and miR-15a/b/16 on wild-type(wt) and mutant(mut) ESRP1 3’UTR. Red dots represented base deletion mutations. e Western blot demonstrated the inhibitory effect of miRs on ESRP1. Histogram represented quantification of bands. f qRT-PCR verified 10 circRNAs in normal ovarian, primary ovarian cancer and metastatic carcinoma tissue. And the binding sites of circ-0005585 to miRs. The official name of circ5 in circBase was obtained by sequence matching. circ5 RNA-Seq subject ID was chr5_43704400_43675511_ + 28889-NNT. CircBase ID was hsa_circ_0005585. *P<0.05, **P<0.01, ***P<0.001. Error bars indicate SEM