Fig. 3
From: High expression of ESRP1 regulated by circ-0005585 promotes cell colonization in ovarian cancer
 circ-0005585 competitively bound miRs to up-regulate ESRP1. a Clinical samples validation of circ-0005585 expression in BOT (Benign Ovarian Tissue, n = 10) and EOC (Epithelial Ovarian Cancer, n = 10). b circ-0005585 DNA sequencing, red arrow represented circulation direction. c Actinomycin D was added in A2780 and SKOV3 cells to inhibit transcription. Then the stability of circ-0005585 was verified by comparing with line-0005585. d Western blot showed that circ-0005585 promoted ESRP1. Histogram represented quantification of bands. e circ-0005585 was transfected into HO8910 cells alone or co-transfected with miRs to examine its effect on ESRP1 by Western blot. Histogram represented quantification of bands. f RNA immunoprecipitation determined the sponge absorption of circ-0005585 on miR-15a/15b/16 and miR-23a/23b. *P<0.05, **P<0.01, ***P<0.001. Error bars indicate SEM