Fig. 5

 ESRP1 was associated with immunesuppression in tumor microenvironment. a Heatmap showed the RNA-Seq differentially genes in ESRP1-SKOV3 and EV-SKOV3. b Volcanoplot indicated the distribution of differential genes by RNA-Seq. Blue dots indicated downregulation (log₂FC < −-1, FDR < 0.05) in ESRP1-SKOV3. Red dots indicated upregulation (log₂FC > 1, FDR < 0.05). c KEGG pathway analysis of differential genes screened by RNA-Seq. d ESRP1/EV-ID8 cells were injected intraperitoneally into C57 mice. Red circles marked macroscopic tumor tissue. e Ascites from two groups of C57 mice were collected, and the amount of CD8 + T cells was detected by flow cytometry. f ESRP1/EV-ID8 cells were injected subcutaneously into C57 mice. Red circles marked tumor tissue, and the histogram showed tumor weight. g Immunohistochemistry was used to detect the expression of ESRP1, CD8 and INHBE in subcutaneous nodules from C57 mice. Scale bar 50 μm. *P<0.05, **P<0.01, ***P<0.001. Error bars indicate SEM