Fig. 9
Effect of PKM2 on cell proliferation in bladder cancer cells. a PKM2 and PKM1 expression in T24 and 5637 cells transfected with siRNAs determined by Western blot. Data are presented as the mean ± SD, n = 3, **p < 0.01 and ***P < 0.001. b Cell growth was detected by CCK8 assay in T24 and 5637 cells after transfection with NC or PKM2 siRNA. Data are presented as the mean ± SD, n = 3, *p < 0.05 and **P < 0.01 vs NC. c, d Cell proliferation was detected using the EdU assay in T24 and 5637 cells (100× magnification). Data are presented as the mean ± SD, n = 3, *p < 0.05. e Flow cytometric analysis of the cell cycle distribution in T24 and 5637 cells post-transfection with NC or PKM2 siRNA. The percentages of cells in G0/G1, S and G2/M phase are shown in the bar graph. Data are presented as the mean ± SD, n = 3, **p < 0.01. NC, negative control. si-1, PKM2 siRNA-1. si-2, PKM2 siRNA-2