Fig. 3

Circ-MAT2B sponges miR-515-5p in GC cells. a RIP assay in AGS and MKN45 cells using anti-Ago2 antibody, followed by qRT-PCR analysis of circ-MAT2B expression. b The indicated three online tools predicting six miRNAs bound by circ-MAT2B. c RNA pull-down in AGS and MKN45 cells using biotin-labeled circ-MAT2B probe, followed by qRT-PCR analysis. d The sketch showing the luciferase reporter assay using wild-type or mutant circ-MAT2B vector. e The luciferase reporter assay in AGS and MKN45 cells co-transfected with miR-515-5p mimics and the indicated luciferase circ-MAT2B vector. f, g qRT-PCR analysis of miR-515-5p expression in circ-MAT2B knockdown GC cells or in GC tissues. (h, i) The indicated functional assays in circ-MAT2B knockdown GC cells transfected with miR-515-5p inhibitors. *p < 0.05, **p < 0.01, ***p < 0.001