Fig. 1

NRP1 is highly expressed in GC and associated with poor prognosis. a The mRNA expression of NRP1 in 30-paired GC tissues and adjacent normal tissues from GZPH GC cohort was analyzed by qPCR. b The protein expression of NRP1 in 8-paired GC tissues (T) and adjacent normal tissues (N) was analyzed by western blot. c The mRNA expression of NRP1 in GC cell lines (SGC-7901, AGS, MGC-823, MKN-45, MKN-28 and BGC-823) and control cell line GES-1 was analyzed by qPCR. d IHC staining of NRP1 was performed using GC TMA. The representative NRP1 staining with different staining intensity scores was shown. Scale bars, 200 μm. e The distribution of NRP1 IHC staining scores in GC tissues and non-tumor control tissues. (f–h) The distribution of NRP1 IHC staining scores in GC with TNM stages I and II or stages III and IV (f), with absent or present of distant metastasis (g), or with absent or present of recurrence (h). i Kaplan–Meier analysis of the association between OS and GC patients with high- or low- expression of NRP1. j Kaplan–Meier analysis of the association between DFS and GC patients with high- or low- expression of NRP1. *p < 0.05; **p < 0.01; ***p < 0.001