Fig. 3
From: MicroRNA-19b-3p suppresses gastric cancer development by negatively regulating neuropilin-1
Knockdown of NRP1 inhibits GC cell growth, migration and invasion in vitro. SGC-7901 or MGC-823 cells were transfected with si-NRP1 or negative control (NC). a The protein expression of NRP1 was examined by western blot 48 h later. GAPDH was used as an internal loading control. b, c Cell proliferation was assessed by CCK-8 assay at indicated time points. d DNA synthesis was evaluated by EDU/DAPI staining. Scale bars, 50 μm. e Cell growth was assessed by colony formation assay. Scale bars, 8 mm. f Cell migration was assessed by wound-healing assay. Scale bars, 500 μm. g Cell invasion was assessed by transwell assay. Scale bars, 50 μm. *p < 0.05; **p < 0.01