Fig. 4

Knockdown of NRP1 inhibits GC development and progression in vivo. SGC-7901 cells were stably transfected with sh-NRP1 or negative control (NC) and subcutaneously inoculated into nude mice. a The representative image of nude mice inoculated with SGC-7901 cells was shown at week 0 and week 5. b The relative photon flux of GC tumor in nude mice of NC or sh-NRP1 group was analyzed by a live imaging system to measure the luciferase signal. c Tumor growth was monitored every week and tumor volume was calculated. d The weight of tumors from NC or sh-NRP1 group was analyzed. e The representative H&E staining and IHC staining of Ki-67/NRP1 of tumor tissue sections from NC or sh-NRP1 group were shown. Scale bars, 200 μm. f The relative IHC staining intensity of Ki-67 and NRP1 in tumor sections from NC or sh-NRP1 group was analyzed. *p < 0.05; **p < 0.01