Fig. 5

NRP1 is negatively regulated by miR-19b-3p. a The putative binding sequences between miR-19b-3p and 3′-UTR of WT NRP1 was predicted by online database miRBase. b The expression of miR-19b-3p in GC tissues and adjacent normal tissues of GZPH GC cohort was analyzed by qPCR. c Pearson analysis of the association between the expression of miR-19b-3p and NRP1 in GZPH GC cohort. d, e SGC-7901 or MGC-823 cells were transfected with mimics NC, miR-19b-3p mimics, inhibitor NC or miR-19b-3p inhibitor. The mRNA (d) and protein (e) expression of NRP1 was examined by qPCR and western blot after 48 h. (f) HEK293 cells were transfected with miR-19b-3p mimics or negative control, together with luciferase reporter vector containing WT 3′-UTR of NRP1 (WT-NRP1) or mutated 3′-UTR of NRP1 (Mut-NRP1). The relative luciferase activity was analyzed 48 h later. **p < 0.01