Fig. 6
From: MicroRNA-19b-3p suppresses gastric cancer development by negatively regulating neuropilin-1
MiR-19b-3p regulates GC cell growth, migration and invasion via targeting NRP1. SGC-7901 or MGC-823 cells were transfected with negative control (NC), miR-19b-3p mimics, or miR-19b-3p mimics + pcDNA3.1-NRP1. a The expression of NRP1 was analyzed 48 h later. GAPDH was used as an internal loading control. b, c Cell proliferation of SGC-7901 or MGC-823 cells was assessed by CCK-8 assay at indicated time points. d Cell growth was analyzed by colony formation assay. Scale bars, 8 mm. e Cell invasion was analyzed by transwell assay. Scale bars, 50 μm. f Cell migration was analyzed by wound-healing assay. Scale bars, 500 μm. *p < 0.05; **p < 0.01