Fig. 2

LINC01123 regulated miR-663a in TNBC cell growth. a StarBase and DIANA predicted four candidates as upstream lncRNAs of miR-663a. b RNA pull-down assay detected the enrichment of four lncRNAs in miR-663a-biotin probe-precipitated group. c qRT-PCR analysis of LINC01123 expression in TNBC cell lines and MCF-10A cells. d Following the cellular fractionation in TNBC cells, LINC01123 expression from cytoplasm or nucleus was monitored in qRT-PCR. e The interaction sequences between LINC01123 and miR-663a predicted by starBase. f Luciferase reporter assay in TNBC cells co-transfected with LINC01123 reporters (LINC01123-WT, LINC01123-MUT) and miR-663a mimics or NC mimics. g RIP assay showed the precipitation of LINC01123 and miR-663a in Anti-IgG or Anti-Ago2 group. h, i Knockdown efficacy of LINC01123 and miR-663a in TNBC cells determined by qRT-PCR. j, k The impacts of LINC01123/miR-663a axis on TNBC cell proliferation were probed by colony formation and EdU assays. l, m Flow cytometry analysis and TUNEL assay reflected the influence of LINC01123/miR-663a pathway on TNBC cell apoptosis. Error bar meant the SD of data from three independent experiments. **P < 0.01